Nouvelles

DND Biotech
DND Biotech
ROBONOVA is the model of an on-site (on the reclamation site) ex-situ (treatment on a matrix removed from the natural location) technology, which exploits oxidative-aerobic processes operated by microorganisms native to the matrix being treated (soil, sediment), using the organic contaminant as a source of carbon and energy and oxygen as an electron acceptor. These processes determine the removal of organic contaminants by mineralization and partial oxidation, promoting the humification of the matrix and returning an ecologically active soil.
.
The ROBONOVA prototype is equipped with a bioreactor for the growth of microbial strains to be added to the treated soil, a fully automated windrow-turning machine assigned to the conditioning and bio-stimulating of the soil matrix (2 m3), emission (GAC Filter) and leachate monitoring and treatment plants. The ROBONOVA station provides a number of key continuous monitoring opportunities: of gas and micro-dusts emissions (CO2, O2, CH4, VOC) from bioremediation activities, compared to the weekly/monthly probing activities in the bench-scale and field trials areas; of both metabolic activity of microorganisms and ongoing process during remediation, validating the findings of the laboratory scale towards full-scale bioremediation works; of energy and water consumption, leachate and liquid waste. DND Biotech is developing an update version of this prototype (ROBONOVA 2.0), which fits into a container and is fully automated and deliverable on-site, ready to work. It will represent a bridge between laboratory scale and full scale for bioremediation projects.
.
+ info www.dndbiotech.it
.
#dndbiotech #discovernewdirection #weprotectsoil #newfuture #bioremediation #insitu #bioremediation #infield #pilotplant
DND Biotech
DND Biotech
Bio-reactive permeable barrier

The prototype consists of a transparent Plexiglas chamber, divided into communicating rooms, in which experimental soil has been implanted. In the middle of the chamber, a Bio-reactive permeable barrier was installed, consisting of a diaphragm filled with a reagent material and SBP capsules (Small Bioreactor Platforms® realized by BioCastle Water Technologies Ltd) inoculated with microorganisms isolated for their capacity of depletion of organic contaminants. The prototype simulates the slow flow of filtration of the groundwater and the spill of a solution containing petroleum hydrocarbons. In this way, an artificial plume of contamination is replicated and it crosses the reactive barrier transported by the simulated flow of the aquifer.
A natural and modified zeolites mixture in combination with inoculated SBP bio-capsules was tested in this prototype with remarkable results. A synergistic effect of the two components was reported: the zeolites mixture reduced the hydrocarbons’ levels by means of adsorption and the bio-capsules eliminated the remaining contamination by biodegradation.
.
+ info www.dndbiotech.it
.
#dndbiotech #discovernewdirection #weprotectsoil #newfuture #bioremediation #insitu #bioremediation
DND Biotech
DND Biotech
Simulation of in-situ bio-inoculation and bio-stimulation phases for bioremediation of contaminated soil.

The system is a closed automated hydraulic circuit in which six soil units, representing different lithological layers, are subjected to treatment using biological and bio-stimulant solutions. The flow through the columns is set to simulate the Bio-flushing treatment, with flow rates and pressures that can be regulated. The biological treatment that takes place in the Bio-cells is aimed at the depletion of TPHs and PAHs contained in the experimental soil, carried out by autochthonous microorganism inoculum, isolated in soil samples and selected for their ability to degrade recalcitrant molecules, and by stimulated bacteria that are part of the natural ecology of the treated soil. Nano-bubbles are dosed in the columns by means of an air-water mixer and the eluates are purified.
The process is monitored through sensors inserted in various points of the circuit and this allows to adjust the operation of the system so that the conditions remain similar to those in-situ and favorable for bioremediation. Soil and water samples are analyzed every 15 days. Soil chemical analyses are conducted to determine the variation in the concentration of target contaminants, while water analyses have the objective to determine the toxicity of the matrices being treated.
.
+ info www.dndbiotech.it
.
#dndbiotech #discovernewdirection #weprotectsoil #newfuture #bioremediation #PAHs #insitu #bioremediation
DND Biotech
DND Biotech
DND Biotech series: Integrating molecular tools to study and monitor the progress of your bioremediation plan

Part 3_how NGS works

NGS is a technique based on the isolation, sequencing and identification of the DNA of each living organism present in a sample. Samples of soil or water from a contaminated site are treated and analyzed in the lab by NGS. The output data of this analysis is then further processed with bioinformatic tools and converted in taxonomical information on the bacterial population present in the samples. Applied to bioremediation, this means that the bacteria present in a sample of contaminated soil can be identified and studied to predict how they can contribute to the degradation of selected pollutants. The NGS analysis can also be performed on samples taken at different time points along the course of the remediation strategy to assess its progress based on the changes in the bacterial population. If necessary, key parameters can be corrected to benefit the achievement of the desired end results.
.
Keep in touch with DND Biotech!
.
+ info www.dndbiotech.it
.
#dndbiotech #discovernewdirection #weprotectsoil #newfuture #bioremediation #PAHs #monitoringtools #nextgenerationsequencing #molecularanalysis
DND Biotech
DND Biotech
DND Biotech series: Integrating molecular tools to study and monitor the progress of your bioremediation plan

Part 2_what molecular tools are

Next generation sequencing (NGS) is a powerful molecular tool that allows to identify the microbial population present in a sample and characterize its biodegradation abilities. Using bioinformatic approaches, from the data obtained with NGS it is possible to infer the functional capabilities of the bacterial community present in the contaminated soil for biodegradation of selected pollutants. Degradative pathways are identified and assessed in terms of relative abundance within the sample. At the beginning of a remediation project, this information can be used to design the optimal engineering strategy. During the remediation, NGS can provide insights in the progression of the biological degradation.
.
In part 3 we will see how NGS analysis works
.
Keep in touch with DND Biotech!
.
+ info www.dndbiotech.it
.
#dndbiotech #discovernewdirection #weprotectsoil #newfuture #bioremediation #PAHs #monitoringtools #nextgenerationsequencing #molecularanalysis
DND Biotech
DND Biotech
DND Biotech series: Integrating molecular tools to study and monitor the progress of your bioremediation plan.

Part 1_What molecular tools can do.
We believe that a successful bioremediation strategy should include analyses that provide information on the microbial ecology of the contaminated area. Molecular tools can be used prior to the remediation to study the indigenous microbial population and its potential biodegradation capability for the pollutants of interest. In this way, it is possible to better engineer the full-scale remediation plan in order to obtain the required results in the fastest way. Molecular tools can also be applied during the remediation strategy to follow its progress and adjust key parameters if needed.
.
In part 2 we will see what these molecular tools are.
.
Keep in touch with DND Biotech!
.
+ info www.dndbiotech.it
.
#dndbiotech #discovernewdirection #weprotectsoil #newfuture #bioremediation #PAHs #monitoringtools #molecularanalysis