Biomass Production

During our projects, we have verified that the selection of autochthonous microbial strains (strains selected directly from the polluted matrix) is the most efficient way to obtain microorganisms capable of reducing the concentrations of pollutants in bioremediation practices. DND Biotech can deliver customized microbial population, site-specific, to be used for bioaugmentation purposes.

The microbial culture is produced using the matrix (soil or water) of the site to be treated, enriching and isolating the microbial communities and/or strains that are most relevant to the degradation process of the contaminants of interest. This process is possible by first growing the microbial population under similar conditions found at the site and with the contaminants of interest as main or sole energy source for the growth process, adding only phosphorus- and nitrogen-based compounds as nutrients. Secondly, once the competent microbes have been enriched, the culture can be tested in lab and/or field trials and additionally, if required, characterized by a metagenomic approach, identifying the microbial species that compose it. Last, the culture is scaled-up, increasing its microbial concentration and available volumes.

At the end of the process, the culture can be delivered in liquid format to the treatment site to be readily used for bioaugmentation. It is possible to enrich both bacterial and fungal cultures, to be used separately or in combination. For difficult and recalcitrant contaminants, a remediation strategy that combines the abilities of both bacterial and fungal degradation processes can lead to a faster, more effective and more efficient bioremediation treatment.

In some cases, and if allowed by the laws of the country of use, already established allochthonous cultures for specific contaminants readily available from our catalog, can be delivered quickly. We still recommend running preliminary lab or field tests to identify the best operating conditions for a successful application of the allochthonous culture to the specific site.